Clone TS1/22 recognizes a 170-180 kD alpha chain of a type I transmembrane glycoprotein also known as CD11a or LFA-1a. CD11a non-covalently associates with integrin ?2 (CD18) to form LFA-1. CD11a is abundantly expressed on all leukocytes, including B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils but does not express by the non-hematopoietic tissues and platelets. Up-regulation of CD11a on activated lymphocytes is required to induce their immune response. Absence or reduced CD11a expression on tumor cells helps to escape from regular immune surveillance and it may be one of reason of in vivo tumor generation.
Each lot of these antibodies has been pre-titrated and tested by flow cytometric analysis of human PBMCs such that 0.5μg (unconjugated, Biotin) or 5μl (conjugated) of these products are sufficient for staining 1 million cells in a 100μl staining volume or 100μl of whole blood. It is recommended to titrate antibody reactivity empirically for optimal performance. Do you need an antibody for an antigen you don't see on our website? Do you need a different conjugation than what we have listed? We have a wide selection of antigens with a variety of conjugations available. Please reach out to us at [email protected].